Impromii reverse transcriptase enables robust, fulllength cdna synthesis for the reproducible analysis of rare or long messages. Impromii reverse transcriptase product informationpdf. The components of the impromii reverse transcription system can be used to reverse transcribe rna templates starting with. Impromii reverse transcription system from promega. Comparison of reverse transcriptases in gene expression.
Northwestern university evanston products and parts names may be covered by issued or pending patents, please see. Certificate of analysis impromii reverse transcriptase cat. The improm ii reverse transcription system produces efficient, robust synthesis of firststrand cdna in preparation for pcr amplification. Impromii reverse transcriptase product informationpdf 107 kb english. The effect of reverse transcription enzymes and conditions on high. Page 4 revised 1205 m 1g 100ng t1g 100ng 1g 100ng t1g 100ng 1g 100ng t 37c 940bp firststrand cdna completed by impromii rt 8. Impromii reverse transcription system protocol promega. Access rtpcr system technical bulletin, tb220 ispybio. Northwestern university evanston products and parts names may be covered by issued or pending patents, please see for more information.
Amplification of specific rnas using the access rtpcr system. I impromii reverse transcription system enzyme promega, madison, wi. Goscript reverse transcription system ohio university. Reverse transcription system technical bulletinpdf 445 kb english. Description the impromii reverse transcription systema is a convenient kit that includes a reverse transcriptase and an optimized set of reagents designed for e.
The impromii reverse transcription system is a convenient kit that includes a reverse transcriptase and an optimized set of reagents designed. Primer hybridization relies on access to the appropriate target site in the mrna and may vary substantially because of mrna folding 11 12. Promega manufacturing and delivery systems continue to be fully operational. M314a impromii reverse transcriptase, 1lreaction 100l m289a impromii 5x reaction buffer 600l a351h mgcl 2, 25mm 1. Improm ii mastercycler mastertaq molecular bioproducts nunc panvera promega sigmagenosys takara enzymes fisher offers a variety of rt enzymes, including mmlv,amv, rav2, and improm ii promega, plus convenient premixes like cmaster rt and the promega reverse transcription system. The impromii reverse transcription systema is a convenient kit that includes a reverse transcriptase and an optimized set of reagents designed for efficient. Promega manufacturing and delivery systems continue to be fully operational during the covid19 outbreak. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible.
The impromii reverse transcription system from promega offers a reliable way to achieve optimal results in cdna synthesis. Impromiitm reverse transcription system tm236 promega. Laube n, pullmann m, hergarten s, schmidt m, hesse a. To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. L, recombinant rnasin ribonuclease inhibitor 2500u, nucleasefree. Impromii reverse transcription system technical manualpdf 559 kb english. The kit contains everything necessary for cdna synthesis. Jun 14, 2019 improm ii promega pdf admin june 14, 2019 the impromii reverse transcription system is a convenient kit that includes a reverse transcriptase and an optimized set of reagents designed for efficient. Sep 01, 2004 the reverse transcriptases are as follows. Additional administrative changes were made throughout the document. Promegas sole obligation and the customers sole remedy is limited to replacement. Volumes from 1l to 20l of the reverse transcription reaction can be directly amplified using taq dna polymerase in pcr. The effect of reverse transcription enzymes and conditions.
Template and primer mix were heated to 70 c impromii or 65 c. Certificate of analysis improm ii reverse transcriptase cat. Superior performance for all of your rna analysis needs stop rnase before it stops you promega notes number 86 2004 25 abstract over 6,000 papers have cited rnasin ribonuclease inhibitors as the source of rnase protection since promega introduced. Impromii reverse transcriptase promega corporation. Promega impromii reverse transcription complete system 100. Description the impromii reverse transcription systema is a convenient kit that includes a reverse transcriptase and an optimized set of reagents designed for efficient synthesis of firststrand cdna in. Apr 20, 2019 improm ii promega pdf posted on april 20, 2019 by admin the impromii reverse transcription system is a convenient kit that includes a reverse transcriptase and an optimized set of reagents designed for efficient. Base misincorporations that may occur during polymerization. Pdf the effect of reverse transcription enzymes and. Improm ii reverse transcriptase enables robust, fulllength cdna synthesis for the reproducible analysis of rare or long messages. Superscript ii reverse transcriptase 10,000 units total, at 200ul is supplied with a vial of 5x firststrand buffer 250 mm trishcl ph 8.
Impromii reverse transcription system technical manual pdf 559 kb english. Impromii reverse transcription system promega corporation. Helpful for preparing long cdnas and libraries with a high percentage of fulllength cdna. Pfu dna polymerase is a highfidelity, thermostable enzyme of approximately 90kda isolated from pyrococcus furiosus.
A3800 impromii reverse transcription system, 100rxn 732,000 586,000 20% a3802 impromii reverse transcriptase, 100rxn 201,000 161,000 a3803 impromii reverse transcriptase, 500rxn 795,000 636,000 m1701 mmlv reverse transcriptase, 10,000u 102,000 82,000 m1705 mmlv reverse transcriptase, 50,000u 386,000 309,000. Nov 24, 2019 improm ii promega pdf the impromii reverse transcription system is a convenient kit that includes a reverse transcriptase and an optimized set of reagents designed for. Impromii reverse transcription system, promega vwr. Promega corporation 2800 woods hollow road madison, wi 537115399 usa. Gotaq, rnasin, plexor, wizard, dualluciferase, celltiter 96 and pgem are registered. The enzyme replicates dna at 75c, catalyzing the polymerization of nucleotides in the 5.
Sep 06, 2012 the impromii reverse transcription system from promega offers a reliable way to achieve optimal results in cdna synthesis. The impromii reverse transcriptase can be used to reverse transcribe total. Promega products are warranted to meet or exceed the stated specifications. Pcr reagents are kits that include researchersupplied components, such as purified nucleic acid templates and oligonucleotide primers, along with components available through vwr, such as deoxyribonucleotides dntps, and dna polymerase. Promega responded that prior statements by life had created the impression that promega was not required to prove anything at trial regarding the amount. The impromii reverse transcription system produces efficient, robust synthesis of firststrand cdna in preparation for pcr amplification. Impromii reverse transcriptase product information pdf 107.
M314 impromiireverse transcriptase 2 n251 rrnasin rnase inhibitor 1 p119 nucleasefree water 2 c114 dntp mix, 10mm 1 a109 upstream control primer 1 a110 downstream control primer 1 c8 1. The improm ii reverse transcription system is a convenient kit that includes a reverse transcriptase and an optimized set of reagents designed for efficient synthesis of firststrand cdna in preparation for pcr amplification. Mutations in the rnase h domain of the enzyme eliminate degradation of the rna during firststrand. Component size m314c impromii reverse transcriptase, 1lreaction 500l m289c impromii 5x reaction buffer 2. The components of the impromii reverse transcription system can be used to reverse transcribe rna templates. Apr 20, 2020 improm ii promega pdf april 20, 2020 the impromii reverse transcription system is a convenient kit that includes a reverse transcriptase and an optimized set of reagents designed for efficient. Promega impromii reverse transcription formulated for standalone applications or for easy transition to genespecific target amplification. Promega corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, highvalue products for the life sciences. Improm ii reverse transcriptasea is provided in quantities sufficient for 10500 firststrand cdna synthesis reactions of 20l each.
Promega impromii reverse transcription complete system. Invitrogen superscript ii reverse transcriptase is a genetically engineered mmlv reverse transcriptase rt with reduced rnase h activity and increased thermal stability compared to wildtype mmlv rt. Description the improm ii reverse transcription systema is a convenient kit that includes a reverse transcriptase and an optimized set of reagents designed for efficient synthesis of firststrand cdna in. Enzyme for fulllength cdna synthesis for the analysis of rare or long messages.
Impromii mastercycler mastertaq molecular bioproducts nunc panvera promega sigmagenosys takara enzymes fisher offers a variety of rt enzymes, including mmlv,amv, rav2, and impromii promega, plus convenient premixes like cmaster rt and the promega reverse transcription system. United states court of appeals for the federal circuit. Omniscript omni, camv, thermoscript thermo, and superscript iii super. The impromii reverse transcription system is a convenient kit that includes a reverse transcriptase and an optimized set of reagents designed for efficient synthesis of firststrand cdna in preparation for pcr amplification. The impromii reverse transcription systema is a convenient kit that includes a reverse transcriptase and an optimized set of reagents designed for efficient synthesis of firststrand cdna in preparation for pcr amplification. Apr 06, 2020 improm ii promega pdf the impromii reverse transcription system is a convenient kit that includes a reverse transcriptase and an optimized set of reagents designed for efficient.